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Western Blot Film

Autoradiography film has been used for many years to detect radioactive molecules, and more recently, to detect specific nonradioactive molecules using probes that catalyze chemiluminescence. These films are most commonly used today for blotting applications, especially Western Blotting. 

Choosing the right film

Proper film choice is determined by the speed (sensitivity) and resolution required. Speed and resolution are determined by the size of the silver halide crystals and thickness of the photographic emulsion. Larger crystals and thicker emulsions enhance sensitivity, but at the expense of resolution. Finer crystals and thinner layers provide greater detail. Maximum-resolution films are best suited to lower energy β -emitters such as 33 P and 14 C. High-performance films such as BioMax Light are recommended for chemiluminescence and high-energy emitters such as 32 P.

BLUE FILM: Azure-XR requires minimal exposure time and yet produces radiographs with sharp and high contrast. The sensitivity of a blue film is lower than that of Kodak BioMax but it is often adequate for many screening applications where qualitative and fast results are required. Azure-XR is suitable for use with ECL substraits to increase sensitivity and it can be processed in automatic processors or manually by using most common X-ray film developers and fixers. Blue Film is much more cost effiective and is the perferred choice in most research laboratories. 

GRAY FILM: For maximum performance, a more expensive grey- or clear-base film may be preferred. Kodak (now CareStream) BioMax scientific films remain the top-ranked preferred choice for best results in the research community. 


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